“The Impact of Candidate Influenza Virus and Egg-Based Manufacture on Vaccine Effectiveness”

Currently, influenza vaccine manufacturing using embryonated hen’s eggs remains the most common vaccine production method, primarily due to historical factors, the availability of supply, and lower costs.

However, mutations in the haemagglutinin and neuraminidase proteins of influenza vaccine viruses may occasionally occur, during their propagation in eggs. Known as egg-adaptation changes, these substitutions can affect the antigenicity of the virus and cause a potential mismatch between the vaccine and circulating strains, thus reducing vaccine effectiveness.  Nonetheless, direct evidence linking egg-adaptation changes to a negative impact on vaccine effectiveness is limited or disassociated.

Medialis Ltd was approached by Seqirus, one of the world’s largest influenza vaccine companies, to engage with the top experts in the field, utilizing an evidence-based approach, and provide a first formal consensus on the subject.

To assess the impact of egg-based manufacturing on vaccine effectiveness, Medialis first deconstructed the research question into its component principles. These were then reviewed by a panel of ten influenza experts across four European countries, using their expertise. Concurrently, our analyst team collected relevant research evidence from 170 manuscripts that supplemented the findings provided by the experts.

Agreement amongst the expert panel was achieved using the Medialis Jandhyala Method 1; a novel, online, two-stage assessment of proportional group awareness and consensus, able to generate a referenced claim from incomplete or dissociated data.

The panel of experts unanimously agreed that there is a mechanistic basis for reduced vaccine effectiveness resulting from candidate influenza virusvariation due to egg-based manufacturing, particularly in the influenza A/H3N2 strain. 

Through this study, Medialis was able to bridge the gap in evidence with Real World Evidence (expert opinion) and establish the first formal expert consensus on the impact of vaccine mismatch due to egg-based manufacturing.  These findings have now been published, titled The Impact of Candidate Influenza Virus and Egg-Based Manufacture on Vaccine Effectiveness 2”, in the high impact factor journal Vaccine (IF 3.269)

This is an example of the innovative and novel approaches Medialis Ltd are able to use in the generation of evidence, to support commercialisation of our clients’ products.

If you’d like to know more about our credentials and how we can support your portfolio, contact us at [email protected].

  1. Jandhyala R. A novel method for observing proportional group awareness and consensus of items arising from list-generating questioning. Curr Med Res Opin. 2020;36(5):883-893. doi:10.1080/03007995.2020.1734920
  2. Rajaram S, Wojcik R, Moore C, et al. The impact of candidate influenza virus and egg-based manufacture on vaccine effectiveness: Literature review and expert consensus. Vaccine. Published online June 26, 2020. doi:10.1016/j.vaccine.2020.06.021

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